Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.
"Recent advances in Cytometry Instrumentation, Probes and Methods Anja Mittag, Arkadiusz Pierzchalski, and Attila Tárnok Section I. Down-sizing cytometry to ""micro"" dimension Droplet Microfluidics for High-throughput Analysis of Cells and Particles Michele Zagnoni and Jonathan M. Cooper Parallel Imaging Microfluidic Cytometer Daniel J. Ehrlich, Brian K. McKenna, James G. Evans, Anna C. Belkina, Gerald V. Denis, David H. Sherr and Man Ching Cheung Microfluidic Systems for Live Cell Imaging Philip Lee, Terry Gaige and Paul Hung Rise of the micro-machines: microfluidics and the future of cytometry Donald Wlodkowic and Zbigniew Darzynkiewicz Label-Free Resistive-Pulse Cytometry M. R. Chapman and L. L. Sohn Section II. Imaging cytometry Laser Scanning Cytometry and Its Applications: A Pioneering Technology in the Field of Quantitative Imaging Cytometry Melvin Henriksen, Bruce Miller, Judith Newmark, Yousef Al-Kofahi and Elena Holden Analytical capabilities of ImageStream cytometer Ewa K. Zuba-Surma and Mariusz Z. Ratajczak Laser scanning cytometry: capturing the immune system in situ Mairi A McGrath, Angela M Morton and Margaret M Harnett Image cytometry analysis of circulating tumor cells Lori E. Lowes, David Goodale, Michael Keeney and Alison L. Allan Pre-clinical Applications of Quantitative Imaging Cytometry to Support Drug Discovery David L Krull and Richard A Peterson Leveraging Image Cytometry for the Development of Clinically Feasible Biomarkers: Evaluation of Activated Caspase-3 in Fine Needle Aspirate Biopsies Gloria Juan, Stephen J. Zoog and John Ferbas Automation of the buccal micronucleus cytome assay using laser scanning cytometry Wayne R. Leifert, Maxime François, Philip Thomas, Ed Luther, Elena Holden and Michael Fenech Laser Scanning Cytometry of Mitosis:state and stage analysis Tammy Stefan and James W. Jacobberger Section III. Instrumentation, new probes and methods Lasers in Flow Cytometry William G. Telford The use of hollow fiber membranes combined with cytometry in analysis of bacteriological samples Kawiak J, Stachowiak R, Lyzniak M, Bielecki J, Granicka L Guide to red fluorescent proteins and biosensors for flow cytometry Kiryl D. Piatkevich and Vladislav V. Verkhusha Quantum Dot Technology in Flow Cytometry Pratip K. Chattopadhyay Background-free Cytometry Using Rare Earth Complex Bioprobes Dayong Jin Surface Enhanced Raman Scattering (SERS) Cytometry John P. Nolan and David S. Sebba Recent Advances in Flow Cytometric Cell Sorting Geoffrey W. Osborne"
